Correlated conformational fluctuations during enzymatic catalysis: Implications for catalytic rate enhancement

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Correlated and uncorrelated regions in heart-rate fluctuations during sleep.

Healthy sleep consists of several stages: deep sleep, light sleep, and rapid eye movement (REM) sleep. Here we show that these sleep stages can be characterized and distinguished by correlations of heart rates separated by n beats. Using the detrended fluctuation analysis (DFA) up to fourth order we find that long-range correlations reminiscent to the wake phase are present only in the REM phas...

متن کامل

Energetic Effects of Multiple Hydrogen Bonds. Implications for Enzymatic Catalysis

The rate enhancements obtained by enzymes often require greater than 20 kcaVmol of stabilization that is specific to the transition state. Inspection of active sites suggests that enzymes often use multiple interactions with substrate groups undergoing charge rearrangement to help provide this preferential stabilization. We have analyzed the deprotonation, in DMSO, of benzoic acids with one or ...

متن کامل

Rate and Equilibrium Constants for an Enzyme Conformational Change during Catalysis by Orotidine 5′-Monophosphate Decarboxylase

The caged complex between orotidine 5'-monophosphate decarboxylase (ScOMPDC) and 5-fluoroorotidine 5'-monophosphate (FOMP) undergoes decarboxylation ∼300 times faster than the caged complex between ScOMPDC and the physiological substrate, orotidine 5'-monophosphate (OMP). Consequently, the enzyme conformational changes required to lock FOMP at a protein cage and release product 5-fluorouridine ...

متن کامل

Contrast enhancement in polarimetric imaging with correlated noise fluctuations

We compare the measurement precision of a polarimetric camera to that of a simple intensity camera when imaging a partially polarized light-mark embedded in an intense and partially polarized background. We show that the gain in measurement precision while using a polarimetric camera is maximized when the noise fluctuations on the two polarimetric channels are significantly correlated. Further,...

متن کامل

Structural and kinetic analysis of free methionine-R-sulfoxide reductase from Staphylococcus aureus: conformational changes during catalysis and implications for the catalytic and inhibitory mechanisms.

Free methionine-R-sulfoxide reductase (fRMsr) reduces free methionine R-sulfoxide back to methionine, but its catalytic mechanism is poorly understood. Here, we have determined the crystal structures of the reduced, substrate-bound, and oxidized forms of fRMsr from Staphylococcus aureus. Our structural and biochemical analyses suggest the catalytic mechanism of fRMsr in which Cys(102) functions...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Protein Science

سال: 2008

ISSN: 0961-8368

DOI: 10.1110/ps.220101